Skip to main content
. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: Pain. 2021 May 1;162(5):1483–1499. doi: 10.1097/j.pain.0000000000002147

Figure 2. The numbers of CGRP-R and PACAP-R TG neurons were significantly increased by the repeated NTG administration.

Figure 2.

(A) The protocol to measure PACAP- and CGRP-evoked [Ca2+]in increase and the representative traces of PACAP- and CGRP-induced Ca2+ transients in TG neurons.

(B) The percentages of PACAP-R and CGRP-R neurons in TG cultures from female Swiss Webster mice after 5 repetitive i.p. injections of Veh or NTG (n = 4 mice/group; a total of 137 and 140 neurons were measured in vehicle and NTG groups, respectively). **p < 0.01, ***p < 0.001, Fisher’s exact test between Veh and NTG groups.

(C) The percentages of CGRP-R neurons in TG cultures from Veh- and NTG-treated female Swiss Webster mice (n = 3 mice/group; a total of 95 and 131 neurons were measured in Veh and NTG groups, respectively). **p < 0.001, Fisher’s exact test.

(D) The percentages of PACAP-R and CGRP-R neurons in acutely dissociated TG neurons from female Swiss Webster mice without NGF and GDNF exposure (n = 5-6 mice/group; a total of 146 and 192 neurons were measured in Veh and NTG groups, respectively). **p < 0.01, ***p < 0.001, Fisher’s exact test between vehicle and NTG groups.

(E) The percentages of PACAP-R and CGRP-R neurons in DRG cultures from female Swiss Webster mice after 5 repetitive i.p. injections of Veh or NTG (n = 3-4 mice/group; a total of 306 and 430 neurons were measured in Veh and NTG groups, respectively).

(F) The percentages of PACAP-R and CGRP-R neurons in TG cultures from female Swiss Webster mice that received 1 Veh or NTG injection (n = 6 mice/group; a total of 190 and 199 neurons were measured in Veh and NTG groups, respectively).

(G) The percentages of neurons that exhibit CGRP-induced phosphorylation of CREB and p38 proteins in TG cultures from female Swiss Webster mice after 5 repetitive i.p. injections of Veh or NTG. Neurons were incubated with 100 nM CGRP for 30 min (n = 4 mice/group; a total of 1418 and 1372 neurons were quantified in Veh and NTG groups for pCREB staining; a total of 1216 and 1065 neurons were quantified in Veh and NTG groups for pp38 staining, respectively). ***p < 0.001, Fisher’s exact test between Veh and NTG groups.