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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: Pain. 2021 May 1;162(5):1483–1499. doi: 10.1097/j.pain.0000000000002147

Figure 4. The number of TG neurons that respond to both CGRP and PACAP is increased by repeated NTG administration.

Figure 4.

(A) The percentages of PACAP-R neurons in TG culture from CGRPαEGFPf/+ mice after 5 repeated injections of vehicle or NTG (n = 5 and 6 mice/group in male mice; a total of 226 and 237 neurons were measured in vehicle and NTG groups; n = 5 and 6 mice/group in female mice; a total of 171 and 232 neurons were measured in vehicle and NTG groups, respectively). *p < 0.05, **p < 0.01, Fisher’s exact test between vehicle and NTG groups.

(B) The percentages of PACAP-R neurons in TG culture. Data from male and female mice in A are pooled and grouped by the number of experiments (n = 7). Each experiment included 1-2 vehicle- and NTG-treated littermates of the same sex. ***p < 0.001, two-tailed t-test.

(C) Venn diagrams of the percentages of CGRP-R and PACAP-R neurons in TG culture from female Swiss Webster (same neurons as in Figure 1A) as well as female and male CGRPαEGFPf/+ mice (same neurons as in A).