Skip to main content
. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: Pain. 2021 May 1;162(5):1483–1499. doi: 10.1097/j.pain.0000000000002147

Figure 7. Repeated NTG administration increases the number of PACAP-R TG neurons but not the number of CGRP-R neurons in CGRPα KO mice.

Figure 7.

(A-C) The percentages of CGRP-R (A), PACAP-R (B) and CGRP-R&PACAP-R (C) neurons in TG cultures from female WT or CGRPα KO mice on C57BL/6J background after 5 repeated injections of Veh or NTG (n = 3-6 mice/group, a total of 116, 130,194 and 176 neurons were measured in WT+Veh, WT+NTG, KO+Veh and KO+NTG groups, respectively). *p < 0.05, **p < 0.01, ***p < 0.001, χ2 test followed by post hoc Fisher’s exact test with Bonferroni correction.

(D) Venn diagrams of the percentages of CGRP-R, PACAP-R and EGFP+ neurons in TG cultures from Veh- or NTG-treated CGRPα KO mice (same neurons as in A-C).

(E-F) The percentages of EGFP+ neurons that are CGRP-R&PACAP-R (E) or CGRP-NR&PACAP-NR (F) in TG culture from heterozygous (HZ) CGRPαEGFPf/+ mice (same neurons as in Figure 5B) and CGRPα KO mice (same neurons as in A-C). *p < 0.05, **p < 0.01, ***p < 0.001, χ2 test followed by post hoc Fisher’s exact test with Bonferroni correction.