Figure 3: CASK-KO mice show increased Ca leak.

A) Western blots and mean data of CaMKII T286 (pT286) phosphorylation (normalized to CaMKII-expression), and CaMKII T305-phosphorylation in ventricular homogenates from mice that were harvested 30 min after intraperitoneal injection of ISO (2 mg/kg body weight). Left-panel: data are not normally distributed (D’Agostino-Pearson test) and Mann-Whitney-Test was applied; right panel: data are normally distributed (Shapiro-Wilk-test) and Student’s unpaired t-test was applied. n=mice. B) Western blots and mean data of RyR2 serine 2814 (pS2814) phosphorylation (normalized to RyR2-expression) in ventricular homogenates from mice that were harvested 30 min after intraperitoneal injection of ISO (2 mg/kg body weight). Data are normally distributed (Shapiro-Wilk test) and unpaired t-test applied, n=mice. C) Left panel: original line scans analyzing elementary SR Ca release events (Ca sparks) in resting Fluo-4-loaded ventricular myocytes. Center panel: violin plot of SR Ca spark frequency. n=cells. Data are not normally distributed (D’Agostino-Pearson test), and Kruskal-Wallis-test (p<0.0001) with Dunn’s post-test (p in graph) was applied. Right panel: mean data for Caffeine transient amplitude. Data are normally distributed (Shapiro-Wilk-test) and two-way ANOVA was applied (p for substance p<0.0001 and for genotype p<0.0243). Multiple comparisons were done by Newman-Keuls post-test (p in graph). n=cells. D) Ca transient amplitude at 1 Hz before and after exposure to ISO (10-7 mol/l) in Fluo-4 –loaded isolated ventricular myocytes. Data are not normally distributed (D’Agostino-Pearson test) and *Kruskal-Wallis-test (p<0.0001) was applied. Multiple comparison by Dunn’s post-test (p in graph). n=cells.