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. Author manuscript; available in PMC: 2022 Apr 16.
Published in final edited form as: Circ Res. 2021 Feb 17:10.1161/CIRCRESAHA.120.318170. doi: 10.1161/CIRCRESAHA.120.318170

Figure 5: CASK-KO accelerates heart failure progression and increases mortality upon TAC.

Figure 5:

A) Original traces of echocardiographic M-mode acquisitions (parasternal long axis view) in CASK-CTRL or CASK-KO mice before TAC, and at two weeks after TAC operation. B) Mean data of left ventricular ejection fraction (EF). Data follows normally distribution (Shapiro-Wilk-test, *two-way repeated measured mixed-effects analysis p for TAC=0.0459 with Holm-Sidak post-test (p values in graph, n=mice). C) Kaplan-Meier survival analysis for CASK-CTRL, CASK-Flox and CASK-KO mice upon TAC. Data were tested by Log-rank (Mantel-Cox) with p in graph. n=mice at risk. D) Mean densitometric expression of CASK (normalized to GAPDH, western blot) in left ventricular homogenates of mice after TAC (vs. Sham-operated mice). Data are normally distributed (Shapiro-Wilk-test) and tested by unpaired t-test (p in graph, n=mice. E) Original registrations (western blot) and mean densitometric data for CaMKII expression and T286 autophosphorylation in ventricular homogenates from CASK-CTRL and KO mice 2 weeks (2w) after TAC. Left panel: Data are not normally distributed (Shapiro-Wilk) and tested by *Kruskall-Wallis p=0.0089. Multiple comparison were done by two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli (p in graph, n=mice. Right panel: Data are normally distributed (Shapiro-Wilk-test) and tested by *ANOVA p=0.0132 with Holm-Sidak post-test (p in graph, n=mice). F) Mean densitometric data for T305-autophosphorylation shows that compared to CASK-CTRL, T305 autophosphorylation was significantly reduced in CASK KO upon TAC. Data are normally distributed (Shapiro-Wilk-test) and compared by unpaired t-test (p in graph, n=mice). G) Mean densitometric analysis of CaMKII-oxidation relative to CaMKII expression indicates enhanced CaMKII oxidation in CASK KO upon TAC. Data are normally distributed (Shapiro-Wilk-test) and compared by unpaired t-test (p in graph, n=mice. Label information: CASK-CTRL = CTRL, CASK-Flox = Flox, CASK-KO = KO