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. Author manuscript; available in PMC: 2022 Apr 16.
Published in final edited form as: Circ Res. 2021 Feb 1;128(8):1191–1204. doi: 10.1161/CIRCRESAHA.120.318562

Figure 3. Blunted frequency-dependent augmentation of contractility in ACi myocytes was improved by MCU overexpression.

Figure 3.

A) Representative recordings of sarcomere length and cytosolic Ca2+ transients with 1, 2, or 4hz stimulation in the absence (Baseline, upper panels) or presence of 100 nM ISO (lower panels) in myocytes of Normal, ACi, ACi+nCFP, and ACi+MCU groups. B) Average sarcomere shortening (%) and ΔR of Fura-2 (340/380nm) measured at 1, 2, or 4hz stimulation with and without ISO in myocytes of Normal (n=10 from 4 hearts), ACi (n=11 from 5 hearts), ACi+nCFP (n=11 from 4 hearts), and Aci+MCU (n=8 from 3 hearts) groups. Effects of stimulation frequency and ISO were analyzed within each group with nested 2-way ANOVA followed with post-hoc Tukey test. Comparisons across groups were performed with nested 2-way ANOVA followed with post-hoc Tukey test under each condition. 1, p=0.0065; 2, p=0.0069; 3, p=0.0070; 4, p=0.0062; 5, p=0.0037; 6, p=0.0064; 7, p=0.012; 8, p=0.0062; 9, p=0.022; 10, p=0.027; 11, p=0.026 compared to Normal group. a, p=0.040; b, p=0.040; c, p=0.044; d, p=0.045 compared to ACi+MCU group.