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. Author manuscript; available in PMC: 2022 Apr 14.
Published in final edited form as: Cell Host Microbe. 2021 Feb 2:S1931-3128(21)00032-9. doi: 10.1016/j.chom.2021.01.002

FIGURE 2. pduC-induced IL-1β mediates AIEC-dependent colitis.

FIGURE 2.

A-B. Germ-free C57BL/6 WT and Il10-deficient mice were colonized with 2×109 CFU of AIEC 2A or pduC-deficient isogenic mutant (2AΔpduC). Ten days after colonization, mice were exposed to 2% dextran sodium sulfate ad libitum for 7 days. Percent weight loss (A) and survival (B) is shown. C,D. C57BL/6 B6 SPF mice were treated with broad spectrum antibiotics for 2 weeks prior to colonization with 1010 CFU of AIEC 2A or 2AΔpduC. Il10, Il23p19 and Il1b expression in the colonic lamina propria mononuclear cells (LPMCs) was measured 5 days after colonization (C). IL-1β production was measured by ELISA in colon explants cultured overnight (D). Graphs show at least 4 mice from one of two experiments. Error bars represent SEM. **p<0.01, ***p<0.005, ****p<0.001 ANOVA. E-G. Germ-free C57BL/6 Il10-deficient mice were colonized with 2×109 CFU of AIEC 2A or 2AΔpduC. Ten days after colonization, mice were exposed to 2% dextran sodium sulfate ad libitum for 7 days. Mice were treated intraperitoneally with anti-IL1β or isotype control on days −2, 0, 2, 4, and 6 of DSS treatment. Weight loss (E), percent survival (F) and levels of lipocalin in cecal contents (G) are shown. Graphs show at least 4 mice from one of two experiments. Error bars represent SEM. **p<0.01, ***p<0.005, ****p<0.001 ANOVA. See also Figure S3.