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. 2021 Mar 8;296:100524. doi: 10.1016/j.jbc.2021.100524

Figure 4.

Figure 4

1,2-NQ induces EGFR internalization.A, cells were incubated with serum-free medium for 3 h and were then stimulated with 10 μM 1,2-NQ for the indicated time. Immunofluorescence staining was performed to visualize EGFR internalization. The scale bar represents 50 μm. B, cells were pretreated with cetuximab in serum-free medium for 3 h and were then exposed to 1,2-NQ for 10 min. Immunofluorescence staining was performed. The scale bar represents 50 μm. C, quantification of EGFR internalization by exposure to 1,2-NQ or EGF for the indicated time. A549 cells were cultured in serum-free medium for 3 h and were then exposed to 1,2-NQ or EGF. 1,2-NQ-treated cells were pretreated with cetuximab in serum-free medium for 3 h. Statistical analysis was carried out by two-way ANOVA with Bonferroni’s multiple comparisons test. The data are expressed as the mean ± SEM values. n = 3, ∗p < 0.05 versus control. D, cells were cultured in serum-free medium for 3 h and were then stimulated with 10 μM 1,2-NQ for the indicated time. Immunofluorescence staining was conducted to visualize the colocalization of EGFR and 1,2-NQ. 1,2-NQ, 1,2-naphthoquinone; EGF, epidermal growth factor; EGFR, epidermal growth factor receptor.