Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 15;11:8279. doi: 10.1038/s41598-021-87441-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

miRNA profiling of exosomes/EVs isolated from sera of CRPC-adenocarcinoma and CRPC-NE (treatment-induced) cases. (A) Representative NTA analyses for CRPC-Adeno (upper left panel) and CRPC-NE case (upper right panel). Particle size (left) and concentration (right) in CRPC-Adeno and CRPC-NE cases as determined by NTA analyses. (B) Western blot analyses for indicated exosomal markers in CRPC-Adeno and CRPC-NE cases. Samples 1–9 and 10–15 were run on two separate gels, with the partitioning indicated by solid black line. Samples derive from the same experiment and gels/blots were processed in parallel. Since CD63 and TSG101 fall in same size range, Western blots were initially probed with CD63 antibody. Following stripping, blots were re-probed with TSG101 antibody. (C) Heat map showing differentially expressed mature EV miRNAs in CRPC-adeno cases (n = 21) as compared to CRPC-NE (n = 6) cases. Heat map was generated by using R studio software, Version 1.1.463 (https://www.npackd.org/p/rstudio/1.1.463). Clusters (I-IV) are denoted by blue bars. (D) PCA plot showing EV-miRNA profiles in CRPC-Adeno and CRPC-NE cases.