Figure 7.

Terpenes Induce CB1-Dependent and Independent Signaling In Vitro. (A) CB1-CHO cells were serum starved for 1 h then pretreated with 10 μM rimonabant or vehicle for 5 min. Cells were then treated with 500 μM terpene, 10 μM WIN55,212-2, or matched vehicle, for 5 min. Lysates were then subjected to Western analysis and blotted for phospho-ERK and total-ERK (see Methods). Graph represents the quantitation of ERK phosphorylation induced by terpene and rimonabant combinations (pERK/tERK). Data expressed as a % of WIN55,212-2 stimulation (n = 3 independent experiments for each). (B) WT CHO cells were serum starved for 1 h then treated with 500 μM terpene, 10 μM WIN55,212-2, or matched vehicle, for 5 min, and analyzed as above. (C) WT CHO cells were serum starved for 1 h, pretreated with 10 μM rimonabant or vehicle, then treated with 500 μM terpene, 10 μM WIN55,212-2, or matched vehicle, for 5 min, and analyzed as above. Data analyzed via one-way ANOVA, Dunnett’s post hoc; **p < 0.01, ****p < 0.0001, compared to vehicle stimulation.