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. 2021 Apr 15;11:8252. doi: 10.1038/s41598-021-87555-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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2-DE analysis of the hippocampus proteome. The timeline of both control and epileptic tissue sampling is shown in each panel. One milligram of total proteins of each sample was separated by 2-DE on a pH 3–10 linear IPG strip in the first dimension and on a 12.5% SDS–PAGE gel in the second dimension. Representative gels from control tissues were showed in (A): the left (A1) and the right hippocampus (A2); panel (B) is related to pilocarpine model: the left (B1) and the right hippocampus (B2); panel (C) showed gels related to PTZ kindling model: The left (C1) and the right hippocampus (C2) and panel (D) showed proteome profile of left (D1) and right (D2) hippocampus related to electrical kindling model. Differently expressed spots were analyzed by ImageMaster 2D Platinum v7.0 and showed by arrows.