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. 2021 Apr 15;11:8252. doi: 10.1038/s41598-021-87555-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Immunoblotting analysis. (A) 2-DE gel images of six protein spots of interest denoted by an arrow in each panel. Each panel shows an expanded 2-D gel view and ranked from the left to the right: the left (CL) and the right hippocampus (CR) of the control group; the left (PTZL) and the right hippocampus (PTZR) of the PTZ group; the left (ElecL) and the right hippocampus (ElecR) of the electrical kindling group; and the left (PiL) and the right hippocampus (PiR) of the pilocarpine group. (B) Western blot analysis validates the differential expression of ADPRC1, Calreticulin, SNAP 25, UCH-L1, LPAR3 and transgelin 3 in the control and the epileptic groups. Beta-actin was used for normalization. (C) The intensity of bands was quantified by ImagJ software. The data were expressed as mean ± S.E.M. The letters above the columns (a-h) indicate significant differences (P < 0.05) using the Duncan’s multiple range test so, data that showed with the same letter don’t have significant differences. (D) Representative photographs of immune-histochemical analysis of the expression levels of the ADPRC1, the calreticulin, the SNAP 25 and the UCH-L1 in control and epileptic groups.