Figure 5.

Blocking of calcium influx inhibits seizure in both in vivo and in vitro seizure models. (A) Suppression of the high-K⁺/low-Mg²⁺ induced epileptiform activity in the CA1 region of the hippocampal slice in the presence of 8-Br-cADPR and 2-APB. Inhibition of epileptiform discharges by reducing of the spiking numbers (B), spiking duration (C), onset latency of spiking (D) and spiking rate (E), in the presence of the both Ca²⁺ channels inhibitors, particularly in the presence of 8-Br-cADPR. 8-Br-cADPR suppresses epileptic seizures in the PTZ-kindled rats (F) as indicated by a significant decrease in seizure duration (G) and an insignificant effect on seizure latency (H). Data were expressed as mean ± S.E.M. Differences between groups were analyzed by one‑way analysis of variance (ANOVA). *Represents a significant difference as compared to control group (P < 0.05).