Massage during reloading (RE) following hindlimb suspension (HS) has no effect on myofibrillar protein turnover, ribosome turnover, or muscle mass or size. Body weight (a), muscle wet weight (b), mean fiber CSA (c), calculated myofibrillar ksyn (d) and myofibrillar kdeg (e), RNA concentration (f), total RNA (g), and calculated RNA ksyn (h) and RNA kdeg (i) from gastrocnemius muscles of WB14 (n = 8), weight bearing for 14 days; HS14 (n = 8), HS for 14 days; RE (n = 10), 14 days of HS followed by 7 days of RE; REM (n = 10), 14 days of HS followed by 7 days of reloading with massage through CCL. Values are mean ± SEM. A one-way ANOVA with a Holm–Sidak post hoc test for multiple comparisons were used to determine statistical significance between groups: *p < 0.05, different from WB14; #p < 0.05, different from HS14