Table 2.

CRISPR technology used for diagnostic method in various countries

Countries	Name of the method	Clinical diagnostic samples	Cas enzyme	Target region	Amplification method	Limit of detection (copy/μL)	Readout method	Sensitivity (%)	Specificity (%)	Total assay time (min)	References
China	CRISPR/Cas12a-NER	31	LbCas12a	E	RT-RAA	10	FL / NE	100	100	45	[43]
China	SENA	295	LbCas12a	N/O	RT-qPCR	< 2	FL	100	100	60	[44]
China	CRISPR-COVID	114	LwCas13a	ORF1ab	RT-RPA	0.3	FL	100	100	40	[14]
Germany	–	24	LwCas13a	ORF1a/N/S	RT-RPA	–	Lf	55.5	100	60	[45]
Japan	CONAN	31	Cas3 and LbCas12a	N	RT-LAMP	< 100	FL/Lf	90	95	40	[46]
Netherlands	DETECTR	378	LbCas12a	N	RT-LAMP	–	FL/Lf	93	95.5	30	[47]
Saudi Arabia	iSCAN	24	LbCas12a and AapCas12b	N/E	RT-LAMP	0.2	FL/Lf	86	100	60	[48]
Thailand	SHERLOCK	154	LwCas13a	S	RT-RPA	2.1	FL/Lf	96	100	55–85	[49]
United Kingdom	–	3	LwCas13a	N	RT-PCR/RT-RPA	0.5	FL	100	NA	75	[50]
USA	DETECTR	82	LbCas12a	N/E	RT-LAMP	10	FL/Lf	95	100	30–40	[51]
USA	STOPCovid	17	AapCas12b	N	RT-LAMP	2	FL/Lf	91.7	100	40–70	[20]
USA	ITP-CRISPR	8	LbCas12a	N/E	RT-LAMP	10	FL	75	100	25	[52]
USA	CRISPR-FDS	35	LbCas12a	ORF1ab/N	RT-PCR/RT-RPA	2	FL	100	71.4	50	[53, 54]
USA	SHINE	50	LwCas13a	ORF1a	RT-RPA	10	FL/Lf	90	100	40	[39, 55]
USA	CREST	1808	LwCas13a	N	RT-PCR	10	FL/Lf	88.8	100	110	[56, 57]