Fig. 3.

ABT 263 and NBE both reduce brain senescence. Freshly frozen 25-μm cryosections of old brains were assayed by SA-βGal, as per manufacturer’s instructions (9860S, Cell Signaling Technology). a Representative images of SA-β-gal staining, scale bar = 50 μm. b Percent SA-β-gal-positive areas per section were quantified and compared between the cohorts. Senescence of the old brain was diminished by both ABT 263 (ABT) and NBE, as compared to the vehicle control (OV) and isochronic old-to-old blood exchanges (OO). p values were obtained by two-tailed Student’s t test. ABT 263 versus vehicle p = 0.0001, NBE versus OO p = 0.0002. N = 4 animals of each cohort. Color coding illustrates the distribution of SA-βGal-positive cells in serial brain sections of each examined animal where the same color represents serial sections of the same brain (circles, vehicle or OO; triangles, ABT 263 or NBE)