Fig. 5.

Multiple determinants of brain health and function become simultaneously elevated after NBE and TPE. a PCA was performed on an aggregate dataset of serum proteomics of control old mice (old exchanged with old) and old animals that underwent one round of NBE (serum was collected 6 days after NBE), with each point representing a unique mouse whose value is computed by a PCA transformation, using as features eleven proteins that play a direct effect in brain health. In this PCA visualization, the aggregation of OO proteome is separated from the aggregation of ONBE proteome. The first two principle components account for 93.5% of the variation between cohorts. B. PCA was performed on human serum proteomics, comparing Pre TPE samples with those collected at 1 month after a single procedure, using as features 17 brain health determinants. The first two principle components account for 88.4% of the variation before and after treatment. C. Heat maps for the loading scores for each principle component for the 17 determinants in human (top) and the 11 determinants in mice (bottom). Erythropoietin and PF4 were evolutionarily conserved proteins influenced by NBE and TPE. All serum samples were the same as in [18].