Table 1.
D2R peptide sequences used for generation of phosphosite-specific antisera.
Antiserum name | Sequence used for immunization | Amino acid position in hD2LR |
---|---|---|
Thr287/Ser288 | EMLSS-T(p)-S(p)-PPER | 282–292 |
Thr293/Ser296 | PPER-T(p)-RY-S(p)-PIPP | 289–300 |
Ser317/Thr318 | HHGLH-S(p)-T(p)-PDSP | 312–322 |
D2R (phosphorylation-independent) |
VNTKRSSRAFRAHLRAPLKGN | 223–243 |
List of peptide sequences used for generating phosphosite-specific antibodies against individual phosphorylated forms of the D2R and a phosphorylation-independent antiserum targeting the D2R at the proximal part of the third intracellular loop.