Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 15;11:8206. doi: 10.1038/s41598-021-87592-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

IL-37 effect on human IEC FliC responses. (a) qPCR analysis of inflammatory genes after FliC and IL-37 exposure for 4 h expressed as percentage of change over FliC treated colonoids. (b) IL-8 and CCL20 protein levels (detected by ELISA) secreted basolaterally by human colonoids after 4 h of stimulation with FliC and IL-37. (c) CCL2, CCL5 and TNFa protein levels secreted basolaterally by colonoids after 4 h of stimulation with FliC and IL-37 (detected by Milliplex Luminex assay). (d) Immunostaining against NFkB (red), actin (Phalloidin—green) and DAPI (blue) of 2D monolayer after 30 min of stimulation with FliC with or without IL-37 (left). Counts of NFkB positive nuclei from immunostaining (right). (e) Western blot analysis of phospho and total p38, after 30 min of stimulation with FliC with or without IL-37 (left). Equal loading confirmed with β-Actin as well as total protein stain of membrane (see Fig. S4 online). Densities relative to total protein or β-Actin are shown (right). Mean and SEM are indicated from n = 4 donors (2 adult and 2 pediatric). All data shown are representative of at least 3 independent experiments. Statistical significance calculated using unpaired Student’s t-test *, P = 0.01 to 0.05; **, P = 0.001 to 0.01; ***, P = 0.0001 to 0.001; ****, P = 0.00001 to 0.0001; ns = not significant.