Table 1.
Primer list for qPCR analysis.
| Target gene | Primer FWD | Primer REV |
|---|---|---|
| Human primers | ||
| CCL20 | GCT ACT CCA CCT CTG CGG CG | ACC TCC AAC CCC AGC AAG GTT CT |
| CXCL2 | GAAAGCTTGTCTCAACCCCG | TGGTCAGTTGGATTTGCCATTTT |
| IL1R | ATGAAATTGATGTTCGTCCCTGT | ACCACGCAATAGTAATGTCCTG |
| IL18R1 | AAGAACGCCGAGTTTGAAGAT | GAGCAGTTGAGCCTTACGTTT |
| IL37 | AAGTCATCCATCCCTTCAGC | CCCACCTGAGCCCTATAAAA |
| IL8 (CXCL8) | TGT GTG AAG GTG CAG TTT TGC | GCA CCC AGT TTT CCT TGG GG |
| SIGIRR | GCTGACTGCAAGGACAGAGA | ACTCGTGGAGGCTGTAGTGG |
| TLR4 | AAGCCGAAAGGTGATTGTTG | CTGAGCAGGGTCTTCTCCAC |
| TLR5 | TGC ATC CAG ATG CTT TTC AG | TGC TGA TGG CAT TGC TAA AG |
| TNFa | TTCCAGAAGATGATCTGATGC | TCAGCCTCTTCTCCTTCCT |
| GAPDH (reference) | ATG ACC TTG CCA CAG CC | CCC ATC ACC ATC TTC CAG |
| TBP (reference) | GCC CGA AAC GCC GAA TAT | CCG TGG TTC GTG GCT CTC T |
| Mouse primers | ||
| Cxcl1 | TGC ACC CAA ACC GAA GTC AT | TTG TCA GAA GCC AGC GTT CAA |
| Cxcl2 | CCT GCC AAG GGT TGA CTT CA | TTC TGT CTG GGC GCA GTG |
| Ccl20 | GCC TCT CGT ACA TAC AGA CGC | CCA GTT CTG CTT TGG ATC AGC |
| Il1r1 | AAGTAATGCTGTCCTGGGCT | AGCACTTTCATATTCTCCATTTGT |
| Tlr4 | TGGCTGGTTTACACATCCATCGGT | TGGCACCATTGAAGCTGAGGTCTA |
| Tlr5 | TGGAGCCGAGTGAGAAATCAG | GTGACGATCCTGGGGTGTTT |
| Sigirr | CCC TGC TCT ATG TTA AGT GTC G | TCA GGT TCA CCA AAA GGT CG |
| Tnfa | CATCTTCTCAAAATTCGAGTGACAA | TGG GAG TAG ACA AGG TAC AAC CC |
| Rplp0I (reference) | AGA TTC GGG ATA TGC TGT TGG C | TCG GGT CCT AGA CCA GTG TTC |
| Gapdh (reference) | ATG ACC TTG CCA CAG CC | CCC ATC ACC ATC TTC CAG |