Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 15;12:2259. doi: 10.1038/s41467-021-22480-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a Experimental scheme for examining the role of SOD1 in KP lung tumor initiation in mice. Blue color indicates Sod1 knockout and red color indicates KP activated. b Quantification of lung tumor burden in Sod1^+/+ KP (n = 10 for 5-week groups and n = 12 for 14-week groups) and Sod1^−/− KP (n = 16 for 5-week groups, and n = 11 for 14-week groups) following tumor induction (administration of adenovirus expressing FlpO (Ad-FlpO)), and 14 and 19 weeks post Sod1 knockout (administration of tamoxifen (TAM)). Data are shown as mean ± SEM (standard error of the mean). Statistical significance was tested using two-tailed unpaired t test, p = 0.0035 (tumor induction in 5-week groups), p = 0.0222 (tumor induction in 14-week groups). c Experimental scheme for examining the role of SOD1 in KP lung tumor maintenance. d Quantification of tumor burden in Sod1^+/+ KP (n = 12 for 12-week groups and n = 10 for 14-week groups) and Sod1^−/− KP (n = 11 for 12-week groups and n = 13 for 14-week groups) post tumor induction (Ad-FlpO administration), and 4-week and 6-week post TAM. Data are shown as mean ± SEM. Statistical significance was tested using two-tailed unpaired t test. n.s., not significant. p = 0.0335 (tumor induction 12-week group), p = 0.0107 (tumor induction 14 weeks group). e Representative images of H&E (hematoxylin and eosin)-stained lung sections from Sod1^+/+ KP and Sod1^−/− KP mice. n = 10 mice per mouse group. Representative data from each group are shown. f Representative H&E staining and IHC staining for SOD1, Ki-67, TUNEL, CC3, and γH2AX in lung tumor sections from 4OHT (4-hydroxytamoxifen)-treated Sod1^+/+ or Sod1^Flox/Flox mice bearing KP tumors. Scale bar, 100 μm. Sod1^Flox/Flox tumors are those occurring in Sod1^Flox/Flox mice that have escaped Cre/LoxP recombination as indicated by SOD1-positive staining. Sod1^−/− tumors are those with Sod1 knockout. n = 3 mice per mouse group. Representative data from each group are shown. g Quantification of Ki-67-positive cells in lung tumors with or without SOD1 (n = 11 for Sod1^Flox/Flox and the Sod1^+/+ group and n = 10 for the Sod1^Δ/Δ group). Sod1^Flox/Flox tumors are counted as part of the Sod1^+/+ group. Data are shown as mean ± SEM. Statistical significance was tested using two-tailed unpaired t test. h Quantification of cleaved caspase-3-positive cells in lung tumors with or without SOD1 (n = 10 for Sod1^Flox/Flox and the Sod1^+/+ group, n = 10 for the Sod1^Δ/Δ group). Data are shown as mean ± SEM. Statistical significance was tested using two-tailed unpaired t test. ns, not significant.