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. 2021 Apr 15;12:2259. doi: 10.1038/s41467-021-22480-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Experimental scheme to generate KP NSCLC cell lines and induce Sod1 knockout by 4OHT. b Genotyping PCR analysis of genomic DNA from Sod1^Flox/Flox KP NSCLC cells following treatment with different doses of 4OHT. Data are represented by three independent experiments (marker unit: bp). c Western blot analysis of SOD1 protein in KP NSCLC cells at different days following treatment with 4OHT. Data are represented by three independent experiments. d SOD1 is required for the growth of KP NSCLC cells. Different Sod1^+/+ and Sod1^Flox/Flox KP NSCLC cell clones (as numbered) were treated with 4OHT overnight, and then cultured for the indicated number of days. Cell growth was measured using the SRB assay. Tetrahydrofuran was used as vehicle control (CTL). Data are represented by means ± SD (standard deviation) of three independent experiments. e Sod1 was knocked down by two independent siRNAs (si-mSOD1) in mouse KP and KL NSCLC cell lines. Cell growth was measured by plate growth assay. “−” is control siRNA. Shown are duplicates for each experiment. f SOD1 was knocked down by siRNA (si-hSOD1) in human KP and KL (KRas-Lkb1) NSCLC cell lines. Cell growth was measured by plate growth assay. “−” indicates control siRNA. Shown are duplicates for each experiment.