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. 2021 Apr 15;12:2259. doi: 10.1038/s41467-021-22480-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, b Flag-SOD1 tagged with an NLS or NES was transiently expressed in Sod1^Flox/Flox KP NSCLC cells treated with 4OHT, and cells were analyzed by Western blot (a) and IF staining (b) (scale bar, 10 μm). Vector, vector control; WT, wild type SOD1; NES, SOD1-NES; NLS, SOD1-NLS; NLS^G93A, SOD1^G93A-NLS; NLS^C147S, SOD1^C147S-NLS. Data are represented by three independent experiments. c Clonogenic proliferation assay of Sod1^Flox/Flox KP cells treated with or without 4OHT overnight, followed by transient expression of different tagged Flag-SOD1. Cells were cultured for 10 days before staining with crystal violet. d Quantification of the colony formation results in (c). Data are represented as means ± SD of three independent experiments.