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. 2021 Apr 15;12:2259. doi: 10.1038/s41467-021-22480-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a–c SOD1 knockdown causes delocalization of BOP1 from pre-rRNA. Mouse NSCLC cells were transfected with mouse SOD1 siRNA (siSOD1) or a control siRNA (siNC). BOP1 and pre-rRNA were stained by IF and RNA-FISH, respectively. Scale bar represents 10 μm. Data are represented by three independent experiments. d–f SOD1 knockdown causes delocalization of BOP1 from pre-rRNA. Human KP and KL NSCLC cells were transfected with human SOD1 siRNA (siSOD1) or a control siRNA (siNC). BOP1 and pre-rRNA were stained by IF and RNA-FISH, respectively. Scale bar represents 10 μm. Data are represented by three independent experiments. g Sod1 knockout impairs pre-rRNA processing. Total RNA was isolated from Sod1^Flox/Flox and Sod1^+/+ KP cells treated with or without 4OHT. Total RNA separated by agarose gel was transferred to a membrane that was stained for 18S/28S total rRNAs by ethidium bromide (EtBr, left panel) and hybridized with a radiolabeled mouse mITS2 probe (right panel). h SOD1 knockdown impairs pre-rRNA processing. Total RNA was isolated from human NSCLC cells (KP: H1155, Calu-1; KL: H2030, A549), human normal lung fibroblasts (WI38), and human normal lung epithelial cells (Beas2B) transfected with human SOD1 siRNA (Sod1) or a control siRNA (nc). RNA separated by agarose gel was hybridized with radiolabeled human probes specific for the human 5′-ETS and ITS2 regions of pre-rRNAs, and 5′-regions of mature 28S and 18S rRNAs. The ratio for 12S/32S and 47S/18S is shown.