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. 2021 Apr 15;11:8276. doi: 10.1038/s41598-021-87677-y

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© The Author(s) 2021, corrected publication 2022

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DNA damage in WBC assessed by comet assay (SCGE); (a,e) show the percentage of DNA in head (integrity) in males and females of the generations F2 (indirect exposed multigenerational lineage) and F3 (transgenerational lineage without partial or indirect exposure) respectively; (b,f) show the percentage of the DNA in tail (fragmentation) in males and females of generations F2 and F3 respectively. Grey bars: control group and linage (n = 24), black bars: As exposed group and linage (n = 24). (*), (**) statistically significant differences intra-gender between control and arsenic exposed group in each generation at p < 0.05 and p < 0.01 respectively. Error bars: standard deviation. (c,g) microphotographs of WBC without fragmentation (F2 and F3, respectively); (d,h) microphotographs of WBC with fragmentation (F2 and F3 respectively).