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. 2021 Apr 15;12(4):407. doi: 10.1038/s41419-021-03675-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Schematic illustration of various FCN3 derivatives: wild type FCN3, FBG domain, SP (signal peptide) + FBG domain, and FCN3 1-90. B Immunoblots showing ectopic expression of various FCN3 derivatives in A549 cells. Antibody against V5 epitope was used. ɑ–Tubulin was used as the loading control. C Expression and subcellular localization of FCN3 derivatives in A549 cells. Nuclear GFP expression indicates the virus-transduced cell. V5 staining shows localization of various FCN3 derivatives. Note no V5 staining in control virus (EV)-transduced cells and nuclear localization of FBG. D Colony formation assay after transducing with control virus or viruses expressing the indicated FCN3 derivatives in A549 cells. Note SP + FBG has growth inhibition effect. Numbers of colonies were counted and presented in bar graphs. Data are mean ± SEM of three independent experiments. E Apoptosis of A549 cells was evaluated with flow cytometry after transducing with control virus or viruses expressing the indicated FCN3 derivatives. (*) and (**) represent P-values of <0.05 and <0.01 from t tests, respectively.