Fig. 1. mRNA codon content predicts translation dependency on U₃₄-enzymes.

a Schematic representation of the dual-luciferase construct. b NanoLuc/Firefly luminescence ratio of MDA-MB231 cells stably expressing the indicated constructs and depleted or not of ELP3 or CTU2 upon IPTG treatment. Data are normalized on non-treated conditions (IPTG 0 μM, n = 3–4 independent experiments, two-sided t-test, data are mean + s.d). c AAA, CAA, and GAA codon frequency and q value were computed for each human gene, red and blue dots represent genes enriched in the three U₃₄-codons (AAA&GAA&CAA q value <0.05 = red dots; AAA&GAA&CAA cumulative frequency >0.088 = blue dots). d qRT-PCR after ribosome immunoprecipitation in MDA-MDMB231 cells. n = 2 independent experiments, two-sided t-test, data are mean + s.d. e Levels of indicated proteins were determined in cells by western blot (n = 1 replicate).