Fig. 6. The presence of hydrophilic motifs discriminates between direct and indirect targets of the U₃₄-tRNA modification pathway.

a Schematic representation of the NDC80 and NUF2 protein sequences. Hydrophilic motifs are indicated in blue. b Protein levels of indicated proteins in cells overexpressing control or NDC80-WT-FLAG or NDC80-Mut-FLAG constructs in the presence or absence of ELP3 or CTU2 (n = 2 replicates). c Protein levels of indicated proteins in cells overexpressing control or NUF2-WT-FLAG or NUF2-Mut-FLAG constructs in the presence or absence of ELP3 or CTU2 (n = 2 replicates). d Levels of indicated proteins were determined by western blot in whole-cell extracts (WCE) and aggregates of MCF7 cells depleted of ELP3 (n = 2 replicates). e Levels of indicated proteins were determined by western blot in cells overexpressing NDC80-WT-FLAG or NDC80-Mut-FLAG constructs in the presence or absence of ELP3 (n = 1 replicate).