Figure 2.

Characterization of C. elegans learning in the maze environment

(A): The effect of maze orientation. Experimental steps are similar to Figure 1E schematic, with testing mazes rotated by 180° (Figure S3), using mazes shown in Figure 1C.

(B): The effect of conflicting environmental cues. Experimental steps are similar to Figure 1E, and an angled maze is shown in inset.

(C): The effect of tactile input. Experimental steps are similar to Figure 1E, with the use of smooth mazes, shown in Figure S1D. All top panels: % of worms scored in Control and Training/Testing experiments (left y axis). Diamond shapes indicate the preference indices (right y axis): for the Control maze Decision Index, for the Training maze Chemotaxis Index, and for the Testing maze Learning Index (Methods). Index error bars represent the standard deviation of the index; dashed line illustrates the 50% and index = 0 level, above bars is indicated the number of nematodes that were scored ($n$), see also Table S2. All bottom panels: p values (binomial distribution probability) for comparisons between each case and basic Control or basic Training/Testing experiment. Control_Basic refers to the basic control experiment (Figures 1D and 1F) and Train_Basic, Test_Basic refer to basic Training/Testing experiment (Figures 1E and 1F).