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. 2021 Mar 17;24(4):102321. doi: 10.1016/j.isci.2021.102321

Figure 3.

Figure 3

Cbl and Cbl-b regulate MAPK signaling

(A and B) SH-SY5Y cells were treated with control siRNA or siRNAs targeting Cbl and Cbl-b for 48 hr prior to stimulation of RTKs with FGF-2, IGF-1, PDGF-BB, or TGF-α as single ligands or a mix (ligand cocktail) for 8 or 90 min. Lysates were immunoblotted using the indicated antibodies as shown in the representative blots (A) and with findings summarized in table (B).

(C) Immunoblotting of lysates from SH-SY5Y cells treated with siGFP or siCbl/Cbl-b and IGF1R inhibitor (NVP-AEW541; + and ++ indicates 250 or 500 nM, respectively) for 72 hr, detecting the indicated proteins.

(D) Representative images based on the same conditions as in (C) (scale bar, 50 μm). Data are representative of three independent experiments (n = 3).