Figure 2. The role of KCNQ1OT1 in ESCC cell proliferation and apoptosis. A. KCNQ1OT1 overexpression plasmid and siRNA targeting KCNQ1OT1 were transfected into ESCC cells. qRT-PCR was used to determine the transfection efficiency, which was compared using Student’s t-test. B, C. The effects of KCNQ1OT1 on ESCC cell proliferation were detected using the CCK-8 method and EdU experiments, and two-way ANOVA and Student’s t-test were used for the comparisons, respectively. D. Flow cytometry analysis was used to detect the effects of KCNQ1OT1 on apoptosis, and Student’s t-test was used for comparisons. **p<0.01 and ***p<0.001. NC, normal control; si-NC, normal control siRNA.