Figure 6. KCNQ1OT1 upregulates EGFR expression by adsorbing miR-133b. A. The TargetScan database was used to predict the binding sites of miR-133b on the 3′-UTR of EGFR. B. A dual-luciferase reporter assay system was employed to detect the binding relationship between EGFR and miR-133b; Student’s t-test was used for comparing the results. C. The effects of KCNQ1OT1 and miR-133b on EGFR expression were detected by qRT-PCR and western blotting, and the results were compared using Student’s t-test. D. Western blotting was used to detect the expression of PI3K/AKT signaling pathway-related proteins; Student’s t-test was used for comparing the results. **p<0.01 and ***p<0.001. WT, wild-type; MUT, mutant; 3′ UTR, 3′ untranslated region.