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. 2021 Apr 16;64(1):37. doi: 10.1186/s13765-021-00608-3

Table 2.

Validation parameters of HPLC

Compound R2 Recovery (%) LOD (mg/kg) LOQ (mg/kg) 5 mg/L 20 mg/L 40 mg/L
RSRr RSDR RSRr RSDR RSRr RSDR
Quercetin 0.9975 87.9 1.14 1.51 0.66 1.89 1.57 1.93 1.81 3.09
Galangin 0.9936 80.0 0.93 1.58 0.72 1.51 0.78 1.67 1.09 1.81
Apigenin 0.9987 104.9 1.13 1.90 0.75 1.01 1.05 1.26 1.16 1.93
Pinocembrin 0.9969 110.2 0.70 1.20 0.78 1.51 1.75 2.27 1.98 2.30
Caffeic acid 0.9983 108.4 0.89 1.45 0.30 1.52 1.22 2.23 1.31 2.93
p-Coumaric acid 0.9980 106.2 0.67 0.93 0.23 1.22 1.32 1.47 1.43 1.68
Trans-ferulic acid 0.9983 98.7 0.56 0.60 0.37 1.12 1.56 1.82 1.78 2.21
Protocatechuic acid 0.9983 108.7 0.51 0.82 0.80 1.18 0.97 1.24 1.07 1.92
Trans-cinnamic acid 0.9984 88.5 0.61 0.66 0.57 1.06 0.91 1.44 1.09 1.76

Caffeic acid

Phenethyl ester (CAPE)

0.9983 98.9 0.51 1.18 0.24 1.37 1.45 1.83 1.59 2.37