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. Author manuscript; available in PMC: 2021 Apr 16.
Published in final edited form as: Cell Rep. 2021 Mar 9;34(10):108822. doi: 10.1016/j.celrep.2021.108822

Figure 3. MED1 enhances MMTV-HER2 tumor cell migration, invasion, and EMT.

Figure 3.

(A and B) H&E staining analyses of tumor lung metastasis in MMTV-HER2 (n = 20) and MMTV-HER2/MMTV-MED1 (n = 18) mice (A), and the quantification (B). Scale bar: 1 mm.

(C and D) Transwell migration (C) and invasion (D) assays of MMTV-HER2 and MMTV-HER2/MMTV-MED1 tumor cells. Scale bar, 100 μm.

(E) Quantification of (C) and (D).

(F) 3D culture acini formation of MMTV-HER2 and MMTV-HER2/MMTV-MED1 tumor cells. Scale bar: 20 μm.

(G) Real-time RT-PCR analyses of the expression of indicated EMT-related genes in MMTV-HER2 and MMTV-HER2/MMTV-MED1 tumors.

(H) Immunofluorescence analysis of E-cadherin, N-cadherin, and Vimentin in MMTV-HER2 and MMTV-HER2/MMTV-MED1 tumor sections. Scale bar: 50 μm. The values are obtained from three independent experiments and shown as mean ± SD. *p < 0.05.