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. 2021 Apr 14;220(6):e202011014. doi: 10.1083/jcb.202011014

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© 2021 Vessoni et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure S4. — Analysis of the effects of ATM or ATRis or CHEK1 and/or CHEK2 silencing on iTERT hPSCs with short telomeres (Tel.). (A and B) Representative histograms of phosphorylated CHK1 (Ser 345) and phosphorylated CHK2 (Thr 68), respectively, in iTERT hPSCs with short telomeres treated with ATMi (10 µm) or ATRi (1 µm) for four consecutive days. (C) mRNA levels of CHEK1 and CHEK2 72 h after transfection with the indicated siRNAs. Bar graphs represent average ± SD of three biological replicates, normalized to housekeeping gene ACTB. (D) Cell proliferation analysis of iTERT hPSCs with long telomeres 7 d after transfection with the indicated siRNAs. Average ± SD of three independent experiments. PDL for iTERT hPSCs with short telomeres: 159.