The PorX/PorY system is the only two-component system essential for the accumulation of iron sources in Porphyromonas gingivalis.A, the growth of 33277 wild-type strain (WT), ΔporX (YS19181), ΔPGN_0775 (YS18343), ΔPGN_0012 (YS18345), ΔPGN_0753 (YS17819), ΔgppX (YS18123), ΔrprY (YS18131), and ΔfimR (YS18069) mutants. B, the growth of 33277 WT with pT-COW (vector) and ΔporX mutant (YS19181) with pT-COW and the complementation plasmid pT-cow-PPGN_1016-porX (labeled as p-porX), respectively. All bacterial strains in (A) and (B) were grown anaerobically on sheep blood BHI agar plates at 37 °C for 7 days.