Skip to main content
. 2021 Mar 21;296:100574. doi: 10.1016/j.jbc.2021.100574

Figure 2.

Figure 2

The receiver and effector domains of the response regulator PorX are sufficient for gene regulation.A, a multiple sequence alignment of the N-terminal sequence of PorX with homologous sequences from TCS response regulators in Escherichia coli K-12. The red color indicates highly conserved columns and blue indicates less conserved ones. Numbering represents the positions of residues in individual proteins. The bottom panel is a model indicating the domains formed in the full-length PorX protein, in which R, E, and ALP represent the receiver domain, effector domain, and alkaline phosphatase-like core domain, respectively. The double arrow indicates the region included in PorX-s (the truncated PorX protein). B, qRT-PCR analysis of transcription of the PorX-activated porT, PGN_0341 and sigH genes. The mRNA level was determined in 33277 wild-type strain (WT), ΔporX mutant (YS19181) with or without plasmid p-porX, and porX-s mutant (YS19363) grown in BHI for 48 h. The mRNA amount in WT was set to 1 for calculation of the relative mRNA level of the porT, PGN_0341 and sigH genes in other strains. Results shown are representative of three independent experiments and all values were mean ± standard deviation and normalized to WT (fold change). ∗p < 0.05, ∗∗p < 0.01, versus wild-type, t-test. C, the growth of 33277 wild-type strain, ΔporX (YS19181), porX-s (YS19363), and ΔporY (YS19187) mutants grown anaerobically on sheep blood BHI agar plates at 37 °C for 7 days.