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. 2021 Mar 21;296:100574. doi: 10.1016/j.jbc.2021.100574

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© 2021 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

P. gingivalis immune responses in a mouse model. DCs and T cells were isolated from mice either unstimulated (PBS) or stimulated on the 7 days with W83 wild-type strain or ΔporX mutant (YS19145) and cultured in the presence of wild-type strain or ΔporX mutant. In total, 25 μl of culture supernatants was collected, and IL-1β (A), IL-6 (B), and IL-10 (C) production from DC and IFN-γ production from T cells (D) were analyzed by Luminex Magpix multiplexing system. These data are expressed as dot plot with the mean ± SD (n = 8 mice). p-value was calculated using a one-way ANOVA (WT group versus ΔporX group). Cells were harvested, stained with directly labeled monoclonal antibodies, and analyzed by FACS analysis. CD4+ and CD8+ T cell populations (E) and CD25+/Foxp3+ regulatory T (Treg) cells (F) were analyzed by flow cytometry.