Figure 2.

Time course of PFV integration to a nucleosome target. A, PFV intasomes with a 40 bp vDNA (vDNA) were incubated with 147 bp Cy5 labeled 601 nucleosomes (NPS). Reaction products were separated by native PAGE stained with ethidium bromide. Left, ethidium bromide image. Right, Cy5 image. Lane 0 is 601 nucleosome substrate only. Lanes 1–6 are 1, 5, 15, 30, 60, and 90 min incubation times. DNA ladder sizes are on the left in bp. B, quantitation of integration over time with Cy5 labeled nucleosomes. The total Cy5 fluorescence in each lane was measured. The percentage of signal is the fluorescent signal excluding the substrate band. Error bars indicate the standard deviation between three independent experiments with at least two PFV intasome and nucleosome preparations.