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. 2021 Mar 18;296:100550. doi: 10.1016/j.jbc.2021.100550

Figure 7.

Figure 7

Affinity of PFV IN truncation mutants for 601 nucleosomes in the presence of a relatively high salt concentration.A, FL, PFV IN(ΔNTD), and PFV IN(ΔCTD) intasomes with biotinylated vDNA were added to 601 nucleosomes and streptavidin-conjugated beads in the presence of 300 mM NaCl. The beads were extensively washed, analyzed by PAGE, and stained with Coomassie brilliant blue. Lane I, 5% of the total proteins. Lane B, proteins associated with beads. Histones H3, H2B, and H2A (H). Streptavidin (S) and histone H4 have the same mobility. B, The total Coomassie signal in each lane was calculated, excluding the band of streptavidin and H4. The fraction of the total signal in each lane B associated with the H3, H2B, and H2A bands was calculated. Black circles indicate values from each experiment. Error bars indicate the standard deviation between three experiments with at least two independent PFV intasome and nucleosome preparations.