Fig. 5.

HDAC inhibitor treatment suppressed the growth of osimertinib resistant (HCC827-OR) and osimertinib sensitive parental (HCC827-P) cells. a, b Photos (a) and growth curve (b) of HCC827-OR and HCC827-P cells. Magnification, 100×. c, d Cells were treated with osimertinib for 3 days and subjected to sulforhodamine B (SRB) assays (c), or were cultured for 24 h and subjected to western blot analysis (d). E, HCC827-OR and HCC827-P cells were treated with TSA (0.5, 1, 2 μmol/L) for 24 h, and subjected to western blot analysis. f, g, HCC827 -OR and H1975-P cells were treated with TSA (f) or vorinostat (g) for 3 days and subjected to SRB assay. Points, mean; bars, SD