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. 2021 Apr 15;21:217. doi: 10.1186/s12935-021-01919-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Alkaline phosphatase (ALPL) inhibits the migration and invasion of LUAD cells by downregulating RhoA protein expression. a Western blot analysis of cell lysates from the indicated cells. b, e Western blot analysis of GFP-RhoA and RhoA expression in A549 (ALPL) and HCC827 (ALPL) cells stably transfected with GFP-RhoA or a vector control plasmid. GAPDH was used as a loading control. Transwell assays were performed to determine the effect of RhoA overexpression on A549 (ALPL) (c) and HCC827 (ALPL) (f) cells, Scale bars: 100 µm. d, g The migration and invasion rate of A549 (ALPL) and HCC827 (ALPL) cells. Data were presented as the mean ± SD and analyzed by Student′s t-test, n = 3. Asterisks (*) indicate a significant increase relative to control vector cells (P < 0.05)