Table 3.
Hands-on time and assay runtime with the examined assays.
| Method | HoT (minutes)1 | Assay runtime (minutes:seconds) |
|---|---|---|
| Genesig Coronavirus (COVID-19) Real-time PCR | Average = 26.05 | 94:00 |
| 95% CI = 24.17–27.92 | ||
| 1Copy | Average = 52.1 | 95:41 |
| 95% CI = 48.34–55.85 | ||
| DNA-Technology | Average = 23.5 | 96:43 |
| 95% CI = 22.27–24.72 | ||
| Charité Primer-Probe sets | Average = 26.625 | 91:07 |
| 95% CI = 25.4–27.85 | ||
| Ustar Isothermal Amplification | < 1 | 79:00 |
| BIOCREDIT COVID-19 Ag rapid test | < 1 | 10−15 |
1
In the case of PCR assays, the hands-on-time was measured for the preparation of 96 samples. In the case of the isothermal amplification and the antigen rapid test, HoT is measured for 1 sample.