Figure 5.
Evaluation of anti-COVID-19 drugs in ACE2-iPS cells
(A) ACE2-iPS cells and Vero cells (2.0×104 cells/well) were infected with SARS-CoV-2 (2.0×103 TCID50/well) in the presence or absence of a drug and then cultured with medium for 4 and 2 days, respectively. The viral RNA copy number in the cell culture supernatant was measured by qPCR.
(B) The EC50 values (μM) of anti-COVID-19 drugs in ACE2-iPS cells and Vero cells were calculated using GraphPad Prism8 and are summarized.
(C) Vero cells and ACE2-iPS cells were cultured with medium containing drugs for 4 days. Cell viability was measured by the WST-8 assay.
(D) The CC50 values (μM) of anti-COVID-19 drugs in Vero cells and ACE2-iPS cells were calculated using GraphPad Prism8 and summarized. Data are represented as means ± SD (n = 3). Unpaired two-tailed Student's t test (∗p < 0.05, ∗∗p < 0.01; ACE2-iPS versus Vero cells).
See also Table S1.