Influence of the conditioned medium of orthodontic bands on the viability of human gingival fibroblasts. Gingival fibroblasts were exposed to the conditioned media of Dentsply-Sirona (DenSir), Dentaurum (Dentaur), TP Orthodontics (TP), and Morelli bands or to the culture medium (control) for 24, 48, and 72 hours, and the number of viable cells was obtained by the vital trypan blue exclusion test. The data represent means and standard deviations. Different letters indicate statistically significant differences (P < .05) among the control and conditioned media from the bands within each time interval.