Table2.
PCR program
| Temperature | Time | Steps |
|---|---|---|
| 95˚C | 5 min | Initial Denaturation |
| 95˚C | 30 sec | Denaturation |
| 59˚C | 50 sec | Annealing |
| 72˚C | 80 sec | Extension |
| 72˚C | 7 min | Final Extension |
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