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. 2021 Apr 13;9:e11076. doi: 10.7717/peerj.11076

Figure 1. Unrooted phylogenetic tree and classification of 364 SmAP2/ERF genes and their representative orthologs from Arabidopsis and Populus.

Figure 1

The amino acid sequences of AP2 domains from 364 SmAP2/ERF proteins and 48 orthologs from Arabidopsis and Populus were aligned by ClustalW, and the neighbor-joining tree was constructed using MEGA 7.0 with 1,000 bootstrap replicates. The evolutionary distances were computed using the p-distance method. In total, 364 SmAP2/ERF members were classified into 15 smaller subgroups, and their names are labeled beside the tree.