Figure 2.

In vitro enzymatic assays of the recombinant CitCHIL1. (a) HPLC analyses of reaction products. The assays were conducted with naringenin chalcone as substrate, and 10 μm protein was added in each reaction. NC, naringenin chalcone; NA, naringenin; EV, proteins of empty pET32a vector. (b) The stereochemistry of recombinant CitCHIL1 catalysed product. HPLC profiles of the reaction products using a chiral separation column. (±) NA, racemic naringenin standard; R, (2R)‐naringenin; S, (2S)‐naringenin. (c) The mass spectra of naringenin standard and reaction products produced by incubation with CitCHIL1.