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. 2020 Dec 3;19(4):830–843. doi: 10.1111/pbi.13510

Figure 4.

Figure 4

FILP and fluorescence spectroscopy measurements of co‐expressed fluorescent protein genes in plants. (a) FILP images of four pairwise combinations of fluorescent proteins synthesized in plants. Bright‐field images for each of the combinations indicates the placement of the three plants with a circled number: (1) vacuum co‐infiltrated, (2) syringe‐infiltrated individual FPs and (3) buffer control. The buffer control is common among combinations. The FILP images were acquired sequentially at 150 ms exposures. (b) Line plots correspond to fluorescence emission measurements taken on agroinfiltrated whole plants for dual fluorescent proteins. Black lines designate the buffer control plant reading for the 1st FP and the buffer for the 2nd FP is in grey. Scale bars for FILP images represent 2.5 cm at a detection distance of 3 m.