Figure 10.

Viral injection controls for CAV experiments, and cognitive deficits seen in LCN-Th-cKO mice are not because of sensory, motor, or anxiety phenotypes. A, CAV-zsGreen injection into LCN (red represents blank; blue represents DAPI; green represents zsGreen). Inset, Coronal schematic of cerebellum at rostral LCN. B, AAV-DIO-GFP injected into LCN of Vglut2-Cre mouse (red represents blank; blue represents DAPI; green represents zsGreen). LatPC, LCN, parvicellular; IntA, interposed cerebellar nucleus, anterior; IntP, interposed cerebellar nucleus, posterior; IntDL, interposed cerebellar nucleus, dorsal hump. A, Inset, Adapted from Franklin and Paxinos (2013). C, Schematic of instrumental learning, and operant-based delayed alternation test for working memory at 8 s, 16 s, and reversal. Adapted from Beas et al. (2017). LCN-Th-cKO mice have no changes in performance on an FR1 instrumental learning (lever training) schedule (D). N = 10 or 11 per group. LCN-Th-cKO mice showed no differences in performance at 8 s (E,I), a small, but significant, reduction in performance on the 16 s version of delayed alternation, as measured by fraction of correct alterations (F) (but not pellets rewarded, J), and no differences in performance on delayed alternation reversal (G,K). N = 7 in each group. H, LCN-Th-cKO mice had a small, but significant, increase in hind stride length (but not stride width) on gait analysis. *p < 0.05 (unpaired Student's t test). **p < 0.01. ns, not significant.